P-55: Effect of Tempol on Sperm Parameters of Diabetic Rats

Background: Diabetes mellitus is an endocrine disorder of carbohydrate metabolism resulting primarily from inadequate insulin release (type I) or insulin insensitivity coupled with inadequate compensatory insulin release (type II). Oxidative stress is one of the complications of diabetes that increases in both types of diabetes and treatment with antioxidants can reduce the complications of diabetes is caused by oxidative. Lipid peroxidation contributes to the damage of the sperm plasma membrane. Tempol is an antioxidant that was applied in studies associated with oxidative stress. Materials and Methods: Wistar albino male rats, 2 months aged and 150-200 g were divided to 4 groups, randomly: group I (control rats without Tempol and STZ), group II (diabetic rats), group III (diabetic rats with Tempol) and group IV (none diabetic rats with Tempol). Rats were done diabetic by STZ injection. After 45 days, the rats were killed and sperm parameters were analyzed.Results: The results obtained in the study revealed that sperm parameters such as count, progressive motility and normal morphology in group II (34 × 106 per ml, 10% and 83.27%, respectively) were reduced rather than group I (57 × 106 per ml, 40.4% and 98.5%, respectively), group III (46 × 106 per ml, 24.6% and 95.34%, respectively) and group IV (60.31 × 106 per ml, 46.5% and 95.8%, respectively), significantly. There were no significant differences between these groups at sperm viability. Conclusion: The present study showed that count, progressive motility and normal morphology in diabetic subjects were reduced, greatly and supplementation diabetic and non-diabetic subjects with tempol antioxidant improved sperm parameters.